Since you brought up Glycerol....Do you think that prehaps sometime in the future, that it might be possible to infuse some kind of anti-freeze mixture into human cells...So that we can be frozen, for say 25 years, and then be defrosted back to life?
Maybe. We already freeze human cells using a chemical called DMSO (dimethyl sulfoxide). It acts for our cells like glycerol does for bacteria - it prevents ice crystals from forming and tearing our cells to shreads.
Its doubtful DMSO would work on a intact human (or even whole organs) as its toxic at the levels required for vitrification (freezing without the formation of ice crystals). Groups have developed vitrificants which potentially can be used to freeze humans, but we're a long ways off of being able to do it. The problems are many, both in the freezing and unfreeing side of things:
1) How do you get the vitrificant into all parts of the body without cutting off O2 flow to those organs
2) How do you deal with the ineviditble toxicity of some of the components
3) How do you keep OO2 flowing to the organs while you freeze?
4) How do you repair parts that don't receive enough vitrificant?
5) How do you thaw and get the vitrificant out without killing the patients
The list goes on and on...
There is also some new evidence from neurobiology that the whole process ma be impossible, at least if you want to retain memories. It appears that the keeping of memories is a dynamic process; i.e. they're maintained by continual interactions between nerves. Its unclear at this point if freezing would stop those from occuring.
Goldfish I believe, have been frozen and brought back to swim again normally.
Some animals have "antifreeze" genes which allow them to survive freezing. This usually requires three features; an antifreeze to prevent ice from forming, a way of regulating metabolism to reduce energy requirements, and a way of maintaining minimal metabolism while "frozen".
Some frogs have this abilities, as do some fish. But they do not "freeze" in the conventional sense; instead their body temps drop below 0C; their hearts still beat, brains still work, etc, thoughbeit at a very low rate.
This "suspended hibernation" may be possible in humans. Hydrogen sulphide can induce a state similar to what is seen in frogs and fish, and mice have been "frozen" to 6C in this manner and recovered without apparent effect. I *think* this H2S-induced hibernation is currently in a phase I trial with humans, although I couldn't find a citation to prove that. I'd point out though, that the goal of this isn't for longevity, but rather is for keeping critically ill patients alive during ambulance transfers, etc.
Bryan