29 March 1999
Then One Bacteria Said To The Other Bacteria�
A research project that started by asking esoteric questions about a glowing marine bacterium has begun to explain the workings of many other bacteria and could result in a new class of antibiotics.
Assistant professor of molecular biology Bonnie Bassler found a gene these bacteria use to sense whether they are part of a dense or sparse population of bacteria -- for example, whether they are living in the human body, as opposed to a puddle of water. And she discovered that more common and more dangerous bacteria have the same gene.
The gene could become a valuable tool to drug developers looking for new ways to attack bacteria that are becoming increasingly resistant to current treatments. In some cases, bacteria only start emitting the toxins that cause disease after they multiply and develop into a dense population. Blocking the effects of the gene could make a bacterium act as though it were back in the puddle.
Bassler found this molecular signal after years of studying certain luminescent bacteria that are widespread in oceans but are harmless to people. Her work focuses on figuring out how and why two species, called Vibrio harveyi and Vibrio fischeri, emit a blue glow.
Bassler and other scientists found years ago that these glowing bacteria are capable of perceiving when they are in a dense population, a phenomenon called "quorum sensing." Each bacterium emits a small signaling chemical that builds in concentration as a population grows. When there is enough chemical, the bacteria adjust to their crowded environment. For V. fischeri and V. harveyi, the response is to emit a blue glow.
In fact, V. harveyi has two quorum-sensing systems, either of which can trigger the glowing. Puzzled about why there would be seemingly redundant systems, Bassler constructed mutant V. harveyi strains that respond to only one signal or the other. She used these mutants to show that one system tells the bacteria how many of its own species are in the area; the other tells how many other types of bacteria are around. That alone was an interesting discovery because it suggested that there is interspecies communication among bacteria. "Vibrio harveyi is out there talking away to these other bacteria," Bassler said.
Perhaps more interesting is the possibility that other bacteria are talking too. In the February 16 issue of the Proceedings of the National Academy of Sciences, Bassler reported that other bacteria produce the chemical that triggers quorum sensing in V. harveyi. She and her collaborators used specially engineered strains of V. harveyi, Salmonella, E. coli, and V. cholerae to show that the gene responsible for signal production was identical in every case. They named the gene LuxS. The discovery of LuxS opened up a whole new avenue of research because scientists can now use it to test in more detail how bacteria use quorum sensing.
One way bacteria may use LuxS could be in controlling their own behavior during infection. For example, Bassler's studies in E. coli and Salmonella suggest that these bacteria may wait until they reach a critical cell number before they produce the virulence factors that cause symptoms of infection. LuxS could be the key to this ability. If bacteria started producing toxins as soon as the infection began, "It would be like waving a flag to alert the host's immune system," Bassler said. "If the bacteria are in small numbers, they don't stand a chance, but if they wait until they reach high cell densities, then they have a much better chance of establishing a productive infection."