Mutating CMV promoter

Posted by
Miryan on Jan 24, 2002 at 16:39 (

The CMV promoter contains an NdeI restriction site, and I want to remove it by site-directed mutagenesis. I will only change one nucloetide (A to T), and I want to know if it can affect the promoter function. Will the promoter stay efficient? The mutation I will do do not change any amino acid (it's a silent mutation)...

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